Composite
Part:BBa_K3081016
Designed by: Yiwen Wang Group: iGEM19_Peking (2019-10-16)
pBAD-dCas9-J23119-M-R2+
This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the linker sequence of two DnaA binding boxes (M and R2) E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth.
Experiment
For more detailed information, see BBa_K3081058
Reference:
[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 5491
Illegal NheI site found at 5514 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1486
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
None |